Antioxidant and Antimicrobial Activity of a Medicinal Mushroom, Ganoderma lucidum (2025)

A detailed comparative study between chemical and bioactive properties of Ganoderma lucidum from different origins

International Journal of Food Sciences and Nutrition, 2014

A detailed comparative study on chemical and bioactive properties of wild and cultivated Ganoderma lucidum from Serbia (GS) and China (GCN) was performed. This species was chosen because of its worldwide use as medicinal mushroom. Higher amounts of sugars were found in GS, while higher amounts of organic acids were recorded in GCN. Unsaturated fatty acids predominated over saturated fatty acids. GCN revealed higher antioxidant activity, while GS exhibited inhibitory potential against human breast and cervical carcinoma cell lines. No cytotoxicity in non-tumour liver primary cell culture was observed for the different samples. Both samples possessed antibacterial and antifungal activities, in some cases even better than the standard antimicrobial drugs. This is the first study reporting a comparison of chemical compounds and bioactivity of G. lucidum samples from different origins.

Extract from wild strain of mushroom Ganoderma lucidum as natural antioxidant

Zbornik Matice srpske za prirodne nauke, 2011

Antioxidant Evaluation of Ganoderma lucidum Extracts

IOP Conference Series: Materials Science and Engineering, 2019

Ganoderma lucidum is one of the many mushrooms utilized by the people as a medicine for cancer, asthma, diabetes and hearth disease. The purpose of this experiment is to determine the antioxidant activity of the extracts from G. lucidum using different solvents. The simplicial powder of G. lucidum are subjected to maceration using water, methanol, ethyl acetate, methylene chloride, and n-hexane; and evaporated the solvent until a viscous extract was obtained. The methanol extract shows highest antioxidant activity with IC50 value of 251.95 μg/mL.

Evaluation of phytochemical , antioxidant , cytotoxicity and in vitro antibacterial activity of aqueous extract of Ganoderma lucidum cultivated in Bangladeshi habitat

2018

Ayurvedic treatment is one of the most ancient treatment systems against various diseases in Indian subcontinent and the western countries have also begun to take this alternative natural therapy as their major treatment system. The medicine of ayurvedic treatment came from various plants or plant parts. Ganoderma lucidum is one of fungus that has been extensively used as various therapeutic agents and contains approximately 400 different bioactive compounds which have been reported to have a number of pharmacological effects. But the biochemical composition of G. lucidium can be changed from its native origin. The biochemical composition, antioxidant activity, cytotoxic activity and antibacterial activity of the G. lucidium cultivated in Bangladesh were detected in this experiment. The phytochemical screening of aqueous extract of G. lucidium was determined using standard methods, the antioxidant activity was evaluated by DPPH free radical scavenging assay, the cytotoxic effect was...

Comparison between antioxidant activity and bioactive compounds of Ganoderma applanatum (Pers.) Pat. and Ganoderma lucidum (Curt.) P. Karst from Iran

2020

Members of Ganoderma genus, belong to Basidiomycota, such as Ganoderma applanatum and Ganoderma lucidum have been noticed in traditional and modern medicine and pharmacology for their medicinal properties and bioactive compounds. The present study is undertaken to determine whether there is difference between chemical properties of G. applanatum and G. lucidum. The fresh mature fruiting bodies of fungi growing on common hornbeam (Carpinus betulus), were collected in Neka, a county in Mazandaran Province, Iran, and their antioxidant activity and bioactive compounds content were examined by spectrophotometer and HPLC method. According to the chemical analysis, the total phenols and flavonoids content, betulinic acid and also antioxidant activity measured by DPPH radical scavenging and FRAP methods in G. applanatum were higher than the other, but G. lucidum had higher content of total polysaccharides and proteins. Also the two other terpenoids (oleanolic acid and ursolic acid) were ver...

In vitro antioxidant, antimicrobial and cytotoxic activities of the various extracts of Ganoderma lucidum available in Bangladesh

Journal of Pharmacognosy and Phytochemistry, 2015

The present study was designed to investigate antioxidant, antimicrobial and cytotoxic potential of pet ether, chloroform and methanol extracts of Ganoderma lucidum available in Bangladesh. Antioxidant activity of the extracts was evaluated using DPPH radical scavenging assay and determination of total phenolic content. Antibacterial and cytotoxic activities were studied using disc diffusion method and brine shrimp lethality bioassay respectively. Results showed that the methanol extract had highest antioxidant activity (IC50 value for DPPH was 47.58 μg/ml and total phenolic content was 167.9±3.252 in mg/g, Gallic acid equivalents) compared to the pet ether and chloroform extract. In antibacterial study, all the extracts showed mild to moderate activity with zone of inhibition ranging from 7 mm to 21 mm. In brine shrimp lethality bioassay, the LC50 values for pet ether, chloroform and methanol extracts were 941.88 μg/ml, 367.28 μg/ml and 295.8 μg/ml respectively which revealed weak ...

Evaluation of antibacterial activity of methanolic and acetone extract of Ganoderma lucidum (Curt.) P. Karst

International journal of applied research, 2016

Medicinal mushrooms are widely used as traditional medicinal ingredients for the treatment of different diseases and related health problems. Ganoderma lucidum provides bioactive compounds that claim to possess antibacterial activity. The fruiting bodies of Ganoderma lucidum were collected from various places in Bilaspur district of Himachal Pradesh, India. The aim of this research is to know antibacterial activity of methanolic and acetone extracts of Ganoderma lucidum. Antibacterial activity of Ganoderma lucidum was done against four human pathogenic bacteria i.e. Yersinia pestis, Pseudomonas aeruginosa, Listeria monocytogenes and Bacillus cereus using agar well diffusion assay. The result showed that the extracts had antibacterial activity and methanolic extract had higher antibacterial activity than acetone extract.

Comparison of antioxidant capacities of two Ganoderma lucidum strains of different geographical origins

Zbornik Matice srpske za prirodne nauke, 2017

Ganoderma lucidum, used in a traditional Chinese medicine, represents one of the most important medicinal mushrooms in the world, whose fruiting bodies and spores have been traditionally used because of a wide spectrum of biological activities such as antidiabetic, antioxidative, antiproliferative, cardioprotective, etc. Its ethnomedicinal importance in some parts of the Balkan region (Serbia and Croatia) is almost totally unknown and there should be more scientific investigations carried out. The aim of this work was to make a comparative study of antioxidative activities and total phenolic content of ethanol and hot water extracts of G. lucidum, collected from forests in Serbia (Morovicke sume, Fruska Gora) and Croatia (Donji Lapac, Pljesevica). The present study was carried out to evaluate antioxidant potential of examined extracts via scavenging potential on ABTS, DPPH, OH? and NO? radicals, as well as of chelating effects via FRAP assay, together with determination of their tot...

Phytochemical Screening and Antimicrobial Activity of Kenyan Mushroom Ganoderma lucidum

Asian Journal of Chemical Sciences

Aim: To screen the Kenyan Ganoderma lucidum for phytochemicals and to investigate it's potential in the management of some disease-causing microbes. Study Design: Hexane, ethyl acetate and methanol extraction; phytochemical study and anti-microbial activities were analysed on Kenyan G. lucidum mushroom. Place and Duration of Study: Masinde Muliro University Science laboratories from January 2019 and March 2019. Methodology: Sequential extraction of dried samples was done using hexane, ethyl acetate and methanol. The crude extracts were assayed against Escherichia coli, Klebsiella pneumoniae, Methicillin-Resistant Staphylococcus aureus (MRSA), Pseudomonas aeroginosa, and Creptococcus neoformans using agar well diffusion method. Ampicillin and nystatin were respectively used as controls for bacteria and fungi. Zones of inhibition measured signified the antimicrobial activity. Results: The following group of compounds were present in the mushroom; terpenoids, carbohydrates, phenoli...

Free Radical Scavenging, Enzyme Inhibitory and Anti-Staphylococcus aureus Properties of the Fruiting Bodies of Ganoderma lucidum, Lingzhi Mushroom

Pharmacology & Pharmacy, 2021

The current study evaluated the antioxidant, α-amylase inhibitory and anti-Staphylococcus aureus activities of the fruiting bodies of Ganoderma lucidum. Methanolic extract of G. lucidum (MEGL) was investigated for its in vitro antioxidant activity using 1,1-diphenyl 2-picrylhydrazyl (DPPH) assay and EC 50 value was determined. α-Amylase inhibitory and anti-S. aureus activities were carried out by conventional methods. The mushroom extract showed maximum free radical scavenging activity at a dose of 500 µg/mL with EC 50 value of 22.1399 µg/mL. EC 50 value for the inhibition of α-amylase activity was found 22.6011 µg/mL. G. lucidum extracts exhibited a concentration-dependent Anti-S. aureus activity. The vast differences of this inhibition between lower and higher doses were assessed significantly (p < 0.05). This study shows that the fruiting body of G. lucidum has moderate antioxidant, α-amylase inhibitory, and anti-S. aureus activities. Thus this mushroom may be the alternative plant source for the antioxidant, anti-diabetic and antibacterial agents and further in vivo studies and isolation of bioactive compounds are required.